Data underlying the manuscript: Lysates of Methylococcus capsulatus Bath induce a lean-like microbiota, intestinal FoxP3+RORγt+IL-17+ Tregs and improve metabolism

Summary This dataset underlies the figures and supplementary figures in the manuscript: “Lysates of Methylococcus capsulatus Bath induce a lean-like microbiota, intestinal FoxP3+RORγt+IL-17+ Tregs and improve metabolism”. The related study showed that exchanging the protein source in a high fat, high sugar, westernised diet (WD) from casein to whole-cell lysates of the non-commensal bacterium Methylococcus capsulatus Bath (McB) was sufficient to reverse WD-induced changes in the gut microbiota to a state resembling that of lean, low fat diet-fed mice, both under mild thermal stress (T22°C) and at thermoneutrality (T30°C). Type of data: gut microbiota and cecal SCFA level changes due to change of the protein source in a high fat, high sugar, westernised diet. Subject of data: Mus musculus Population characteristics: 6-7-week-old male C57BL/6JBomTac, C57BL/6JRj, C57BL/6N, C57BL/6N-Rag2Tm1/CipheRj (RAG2-/-) mice acquired from Taconic Laboratories, Denmark, or Janvier Labs, France. Data The raw data underlying the figures and supplementary figures in the related manuscript are shared openly here as .txt files in tab-separated value format. There are 94 files in total, and each is named in accordance with the particular figure and subfigure it underlies. For more details on the particulars of each file, see the figure captions in the related manuscript. Corresponding author(s) for this study Benjamin. A. H. Jensen, Laboratory of Genomics and Molecular Biomedicine, Department of Biology, Faculty of Science, University of Copenhagen, Denmark. [e-mail]. Karsten Kristiansen, Address as above. [e-mail]. Tor E. Lea , Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Norway. [e-mail]. Study approval All experiments were conducted in accordance with the EU directive 2010/63/EU as approved by the Danish Animal Experiments Inspectorate (#2014-15-2934-01,027).

数据集概述
本数据集支撑了发表于论文《荚膜甲基球菌Bath全细胞裂解物可诱导瘦表型肠道菌群、肠道FoxP3+RORγt+IL-17+调节性T细胞（FoxP3+RORγt+IL-17+ Tregs）并改善代谢》的主图及补充图相关数据。相关研究表明，在高脂高糖西式饮食（westernised diet, WD）中，将蛋白质来源从酪蛋白更换为非共生细菌荚膜甲基球菌Bath（Methylococcus capsulatus Bath, McB）的全细胞裂解物，即可在轻度热应激（22℃）及热中性环境（30℃）条件下，将西式饮食诱导的肠道菌群改变逆转至类似低脂饮食喂养的瘦型小鼠的菌群状态。

数据类型
本数据反映高脂高糖西式饮食中蛋白质来源改变所引发的肠道菌群与盲肠短链脂肪酸（short-chain fatty acids, SCFA）水平变化。

数据对象
数据对象为小家鼠（Mus musculus）。种群特征：6~7周龄雄性C57BL/6JBomTac、C57BL/6JRj、C57BL/6N、C57BL/6N-Rag2Tm1/CipheRj（RAG2-/-）小鼠，购自丹麦Taconic Laboratories或法国Janvier Labs。

数据文件说明
相关论文主图及补充图所依托的原始数据，以制表符分隔值（tab-separated value, TSV）格式的.txt文件形式在此公开共享。共计94个文件，每个文件均根据其所支撑的具体图版及子图命名。关于各文件的详细信息，请参阅相关论文的图注。

通讯作者
本研究的通讯作者如下：
1. Benjamin A. H. Jensen，丹麦哥本哈根大学理学院生物系基因组学与分子生物医学实验室，邮箱：[e-mail]
2. Karsten Kristiansen，通讯地址同上，邮箱：[e-mail]
3. Tor E. Lea，挪威生命科学大学化学、生物技术与食品科学学院，邮箱：[e-mail]

实验伦理审批
所有实验均遵循欧盟2010/63/EU指令，并经丹麦动物实验监察委员会批准（审批号：#2014-15-2934-01、027）。