Factors that affect the transient expression of <i>gusA</i> gene in nodal explants of <i>W</i>. <i>somnifera</i> infected with <i>A</i>. <i>tumefaciens</i> strain LBA4404 containing vector pCAMBIA2301.

Control (0): Treatment without factors aNodal explants from different time duration were pre-cultured on WsSIM and hand-pricked (1–2 times) nodal explants were infected with different Agro culture densities and the explants were co-cultivated for different days on WsSIM medium. bThe effect of AS was evaluated by incorporating it in WsSIM+AS medium. Six-day old pre-cultured nodal explants were infected at OD600 0.2 of Agro culture and co-cultivated for 3 days on WsSIM+AS containing various concentrations of AS. cSix-day old precultured nodal explants were infected in the presence of sonication at different time durations at OD600 0.2 of Agro culture and co-cultivated for 3 days on WsSIM+AS (150 μM). dSix-day old pre-cultured nodal explants were infected in the presence of vacuum infiltration at different time durations at OD600 0.2 of Agro culture and co-cultivated for 3 days on WsSIM+AS (150 μM). eSix-day old pre-cultured nodal explants were infected at OD600 0.2 of Agro culture with a combination of sonication (10 sec) + vacuum infiltration (10 min) and co-cultivated for 3 days on WsSIM+AS (150 μM). fSix-day old pre-cultured nodal explants were infected and sonicated (10 sec) and vacuum infiltrated (10 min) at OD600 0.2 of Agro culture and co-cultivated for 3 days on WsSIM+AS (150 μM) containing various concentrations of thiol compounds. Data were collected at the end of Ist selection cycle on WsSSM-I (15th day). The explants subjected to different factors were placed on WsSIM for 6 days for resting and the explants were transferred to WsSSM-I at 7th day. Factors that affect the transient expression of gusA gene in nodal explants of W. somnifera infected with A. tumefaciens strain LBA4404 containing vector pCAMBIA2301.