Comparison of eSIP parameters using different objectives at a Zeiss LSM 780.

Measurements are conducted using the solution-based calibration sample and an excitation at 440 nm with 5% laser power and pinhole setting 0.5 AU. a A: Amplitude or maximal intensity. The differences in intensity also originate from the big differences in the field of view. Same settings for the zoom result in following field of view sizes. 10x: 1417×1417 μm, 20x: 708×708 μm, 40x: 354×354 μm, 63x: 225×225 μm. All data were measured using 512x512 pixels resulting in different pixel sizes. Comparison of eSIP parameters using different objectives at a Zeiss LSM 780.