Fast Repetition Rate Fluorometry (FRRF) data collected on the RV L'Astrolabe from 22/01/2008 to 27/01/2008 (Dumont d'Urville - Hobart)

These data were collected on the RV L'Astrolabe (platform code: FHZI) from 22/01/2008 to 27/01/2008 on a trip from Dumont D'Urville to Hobart.

Maximum photochemical efficiency of photosystem II (PSII), also called maximum quantum yield of PSII (Fv/Fm), has become one of the most widely utilized fluorescence parameters in phytoplankton research. It represents the potential photochemical efficiency, which is the probability that the light energy captured by the photosynthetic apparatus is being utilized as photochemistry. Fv/Fm has been shown to have an instant response to variations in physical and chemical properties and is interpreted as a diagnostic of the overall health or competence of phytoplankton. Together with the absorption cross section area of PSII and chlorophyll concentration, it can be used to measure primary production (Cheah et al. 2011, Deep Sea Research).

Seawater from 3 m depth was supplied continuously from the ship’s clean seawater line. FRR fluorescence yields were measured continuously at 1 minute intervals in dark-adapted state (! 15 minutes dark-adaptation) using a flash sequence consisting of a series of 100 subsaturation flashlets (1.1 μs flash duration and 2.8 μs interflash period) and a series of 20 relaxation flashlets (1.1 μs flash duration and 51.6 μs interflash period).

本数据集采集于星盘号研究船（RV L'Astrolabe，平台代号：FHZI），采集时段为2008年1月22日至2008年1月27日，航次路线为从迪蒙·迪尔维尔港前往霍巴特。
光系统II（photosystem II, PSII）的最大光化学效率，亦称PSII最大量子产额（Fv/Fm），现已成为浮游植物研究中应用最为广泛的荧光参数之一。该参数表征潜在光化学效率，即光合装置捕获的光能用于光化学反应的概率。研究表明，Fv/Fm可对物理化学性质的变化产生即时响应，常被用于诊断浮游植物的整体健康状态与生存能力。结合PSII吸收截面面积与叶绿素浓度数据，该参数可用于估算初级生产力（Cheah等人，2011年，《深海研究》）。
实验采用3米深度的海水，通过船舶洁净海水管路持续供给。快速重复率荧光（Fast Repetition Rate fluorescence, FRR）产额于暗适应状态（需经15分钟暗适应）下，以1分钟为间隔连续采集；所用闪光序列包含100个亚饱和微闪光（闪光时长1.1 μs，闪光间隔2.8 μs）与20个弛豫微闪光（闪光时长1.1 μs，闪光间隔51.6 μs）。