HPLC-ESI-MS characterization of phytochemicals in Serghina extract

HPLC analysis of serghina (2024) extract was performed on Thermo Fisher Scientific Dionex Ultimate 3000 HPLC System, equipped with the Ultimate 3000 RS Variable Wavelength UV DAD. DAD was performed in-line in UV–VIS spectra in the range of 200 to 800 nm. An Acclaim Polar Advantage column (5µm - C18 - 250 mm x 4.6 mm) was used at 30°C. Data acquisition was performed by Thermo Fisher Scientific Xcalibur software (version 3.1).采用赛默飞世尔科技（Thermo Fisher Scientific）戴安UltiMate 3000高效液相色谱（High Performance Liquid Chromatography, HPLC）系统，对2024年批次的serghina提取物开展分析检测，该系统搭载UltiMate 3000 RS可变波长紫外二极管阵列检测器（Diode Array Detector, DAD）。该二极管阵列检测器在线采集200~800 nm波长范围内的紫外-可见光谱数据。实验采用Acclaim Polar Advantage色谱柱（粒径5μm、C18键合相、规格250 mm × 4.6 mm），柱温维持在30℃。数据采集工作通过赛默飞世尔科技的Xcalibur软件（版本3.1）完成。