10x Single-Cell RNAseq Colon (Withheld Test Set)

Human epithelial, B, and stromal cells from the colon in a SingleCellExperiment object with labels. These data consist of 100 cells withheld from a larger reference set and can be used for evaluating cell-type annotation classifiers. These three different cell-types were combined from the following original studies: Epithelial cells: Wang, Y., Song, W., Wang, J., Wang, T., Xiong, X., Qi, Z., ... & Chen, Y. G. (2020). Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine. Journal of Experimental Medicine, 217(2). B cells: Huang, B., Chen, Z., Geng, L., Wang, J., Liang, H., Cao, Y., ... & Zhang, Y. (2019). Mucosal profiling of pediatric-onset colitis and IBD reveals common pathogenics and therapeutic pathways. Cell, 179(5), 1160-1176. Stromal cells: Kinchen, J., Chen, H. H., Parikh, K., Antanaviciute, A., Jagielowicz, M., Fawkner-Corbett, D., ... & Simmons, A. (2018). Structural remodeling of the human colonic mesenchyme in inflammatory bowel disease. Cell, 175(2), 372-386.