Expression data of BRAF inhibitor resistant melanoma cells and spheroids

In vitro cell cultures are frequently used to define the molecular background of drug resistance. In this study our major aim was to compare the gene expression signature of 2D and 3D cultured BRAFV600E mutant melanoma cell lines. We successfully developed BRAF-drug resistant cell lines from paired primary/metastatic melanoma cell lines in both 2D and 3D in vitro cultures. Using Affymetrix Human Gene 1.0 ST arrays, we determined the gene expression pattern of all cell lines. Our study highlights gene expression alterations that might help to understand the development of acquired resistance in melanoma cells in tumour tissue. We generated spheroids from the BRAFi sensitive (WM983A, WM983B) and BRAFi resistant (WM983ARES, WM983BRES primary/metastatic melanoma cell lines. To analyze the expression profile of the BRAFi resistant cells and BRAFi resistant spheroids, we used for Affymetrix Human Gene 1.0 ST array.