Combinatorial transcriptional regulation of MYBL2 via Ras and ErbB pathway

Transcription factors (TFs) play an important role in tumorigenesis and development. Abnormal transcriptional regulation leads to up-regulation of oncogene expression or down-regulation of tumor suppressor, thus promoting the occurrence and progression of tumors. Chromosomal immunoprecipitation followed by next generation sequencing (ChIP-seq), a transcriptome and genome analysis method, is widely used to identify dysregulated genes and signal pathways in tumors. Transcription factor MYBL2 is overexpressed in numerous tumors and is involved in tumor progression. However, identification of the genes and pathways regulated by MYBL2 warrants further investigation. Here, we investigated the global MYBL2 genomic binding sites using ChIP-seq technology, and annotated its gene function and analyze KEGG pathway. Moreover, the target genes in Ras and ErbB signaling pathway regulated by MYBL2 were validated using RT-qPCR. Through integrated assay, we identified HEB, ZEB1 and ASCL1 as MYBL2 coregulator genes. Taken together, this study provides a reference for a better understanding of MYBL2 regulatory mechanism in tumorigenesis.