Multiple mitochondrial fatty-acid β-oxidation genes regulated by NHR-49 are essential for the increased longevity of germline-less animals but not wild-type worms.

glp-1 mutants (1A) and the sterile strain, fer-15(b26);fem-1(hc17), (1B) were subjected to ‘adult-only’ RNAi inactivation of mitochondrial β-oxidation genes whose expressions were elevated upon germline loss, many in an NHR-49-dependent manner. See methods section for experimental details and S6 Table for additional trials.
*fer-15(b26);fem-1(hc17) is a temperature sensitive strain that when grown at 25°C is sterile and used as a surrogate for wild-type, N2 in lifespan assays [69].
**ech-1.2 (T08B2.7) is orthologous to the human gene that encodes the tri-functional protein, hydroxyl acyl CoA dehydrogenase/3-ketoacyl CoA thiolase/enoyl CoA hydratase (HADHA) alpha subunit (www.wormbase.org) and a close paralog of ech-1.1. ech-1.1 and cpt-5 could not be tested due to contamination of RNAi clones. Data is shown as mean lifespan in days (Mean) ± standard error of the mean (SEM). ‘n’ refers to the number of worms observed (obs) divided by total number of worms tested in the experiment.
asome worms were censored from the analysis as described in methods.
bEmpty vector control refers to worms exposed to empty vector plasmid without an RNAi insert. P values were calculated using the log rank (Mantel Cox) method.
Multiple mitochondrial fatty-acid β-oxidation genes regulated by NHR-49 are essential for the increased longevity of germline-less animals but not wild-type worms.