Characterization of European Sea Bass transcripts by RNA SEQ after Oral Vaccine against V. anguillarum

In the present study we performed RNA seq in two different tissues; gut and head-kidney. For each tissue one control sample (where a sample presents a pool of 4-5 individuals) and one sample after oral vaccine against V. anguillarum were submitted to 454 next generation sequencing. Synthesis of first strand cDNA was performed using oligo nucleotide primer 5’-TTTTTTCTTGTTTTCTTTTCTTV-3’ and cDNA library protocol of GSFLX454 was adapted accordingly.