Dataset on porcine retinal cells after the optic nerve transection.

Figure 1. Retinal morphology and apoptosis after organ culture of porcine retinas. (A) Histological evaluation of cultured retinas. Porcine retinas were cultured in Dulbecco’s modified Eagle’s medium for the indicated times. Cell nuclei were stained with hematoxylin and the cytoplasm with eosin. Scale bar = 50 μm. (B) TUNEL assay of retinal sections after organ culture. TUNEL-positive cells are shown in green. Nuclei are stained blue with 4'-6-diamidino-2-phenylindole (DAPI). As a positive control, retinas were cultured in the presence of 5 mM sodium glutamate for 12 h. Scale bar = 50 μm. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Figure 2. Immunohistochemistry of retinal cell markers after organ culture. Antibodies against rhodopsin (green, panels a-d), M-opsin (red, panels e-h), NeuN (green, panels i-l), glutamine synthetase (red, panels m-p), and GFAP (red, panels q-t) were used. Nuclei are stained blue with DAPI. Scale bar = 50 μm. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; NeuN, neuronal nuclei; GFAP, glial fibrillary acidic protein.图1 猪视网膜器官培养后的视网膜形态与细胞凋亡。(A) 培养视网膜的组织学评估。将猪视网膜在达尔伯克改良伊格尔培养基（Dulbecco’s modified Eagle’s medium）中培养至指定时长，细胞核经苏木精染色，细胞质经伊红染色，比例尺为50 μm。(B) 器官培养后视网膜切片的末端脱氧核苷酸转移酶介导的dUTP缺口末端标记测定（TUNEL）。TUNEL阳性细胞呈绿色，细胞核经4',6-二脒基-2-苯基吲哚（DAPI）染为蓝色。阳性对照组为将视网膜置于含5 mM谷氨酸钠的培养基中培养12 h，比例尺为50 μm。缩写注释：ONL，外核层（outer nuclear layer）；INL，内核层（inner nuclear layer）；GCL，神经节细胞层（ganglion cell layer）。 图2 器官培养后视网膜细胞标志物的免疫组织化学检测。使用针对视紫红质（绿色，图a-d）、视蛋白M亚型（红色，图e-h）、神经元核抗原（NeuN，绿色，图i-l）、谷氨酰胺合成酶（红色，图m-p）以及胶质纤维酸性蛋白（GFAP，红色，图q-t）的抗体。细胞核经DAPI染为蓝色，比例尺为50 μm。缩写注释：ONL，外核层；INL，内核层；GCL，神经节细胞层；NeuN，神经元核抗原（neuronal nuclei）；GFAP，胶质纤维酸性蛋白（glial fibrillary acidic protein）。