Analysis of kinetics of expression of the miRNA-124 precursor transcripts pri-miRNA-124.1, pri-miRNA-124.2 and pri-miRNA-124.3 in RAW264.7 cells treated with IL-4¹.

in Materials and Methods. Data of four experiments performed in triplicates for each precursor is shown.1 The cells were treated with 50 ng/ml of IL-4 and harvested at indicated time points after adding of IL-4. RNA was isolated and expression of pri-miR-124.1, pri-miR-124.2 or pri-miR-124.3 was analyzed by real-time RT PCR as described “−”) if no product was detected after more than 40 cycles of amplification.2 Expression was considered negative ( “+”) for CT<35 cycles.3 Expression was considered positive (