RNase J is required for processing of a small number of RNAs in Rhodobacter sphaeroides
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https://www.ncbi.nlm.nih.gov/sra/SRP036835
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资源简介：
A comparative RNA-seq approach between R. sphaeroides 2.4.1 wild type and an RNase J deletion strain 2.4.1?rnj revealed the accumulation of different mRNA fragments in the mutant. The structural characteristics of these RNA fragments suggest that RNase J is responsible for the decay of degradation intermediates that cannot serve as substrates for the 3´-to-5´ exoribonucleases. Overall design: Total RNA from exponentially grown cultures (OD660 0,4) of 2.4.1 wild type and 2.4.1?rnj has been isolated in triplicates using the hot phenol method and prepared for sequencing on a Illumina Genome Analyzer IIx machine.
创建时间：
2021-08-01
