Data from: Delivered complementation in planta (DCIP) enables measurement of peptide-mediated protein delivery efficiency in plants

Using DCIP, we demonstrate cell-penetrating peptide-mediated cytosolic
delivery of peptides and recombinant proteins in Nicotiana benthamiana. We
show that DCIP enables measurement of delivery efficiency and enables
functional screening of cell-penetrating peptide efficacies for in-planta
protein delivery. Finally, we demonstrate that DCIP detects
cell-penetrating peptide-mediated delivery of recombinantly expressed
proteins such as mCherry and Lifeact into intact leaves. We also
demonstrate delivery of a recombinant plant transcription factor, WUSCHEL
(AtWUS), into N. benthamiana. RT-qPCR analysis of AtWUS delivery in
Arabidopsis seedlings also suggests delivered WUS can recapitulate
transcriptional changes induced by overexpression of AtWUS. Taken
together, our findings demonstrate that DCIP offers a new and powerful
tool for interrogating cytosolic delivery of proteins in plants and
highlights future avenues for engineering plant physiology.