Modeling the effects of acute and chronic oxidative stress on blood-brain barrier phenotype and function in 2D and 3D

We report changes in gene expression of iPSC-derived brain microvascular endothelial cells (iBMECs) exposed to chronic (100 µM continuously) and acute (500 µM for 1 h) hydrogen peroxide over the course of seven days. Overall design: 24 total samples collected; n =4 samples of acute oxidative stress at day 0 and its corresponding control, acute oxidative stress at day 4 and its corresponding control, and chronic oxidative stress at day 7 and its corresponding control