Characterisation of glycation percentage in the different erythrocyte preparations by mass spectrometry.

Data for the four main peak of the mass spectra corresponding to α-hemoglobin (α-Hb; 15130 Da), glycated α-hemoglobin (g-α-Hb; 15330 Da), β-hemoglobin (β-Hb; 15890 Da), glycated β-hemoglobin (g-β-Hb; 16100 Da) (S1 Fig—see supplemental data). G0, G5, G25 and G137 represent the four conditions of incubation to which erythrocytes were subjected: 0, 5, 25 and 137 mmol/l glucose, respectively. Δmass and % glycation were calculated as explained in the methods section. Results are the mean ± SD and statistical analyses were performed using Tukey’s post hoc analysis following a significant one way ANOVA: ***p