Summary of germline transmission with IKMC lines.

Summary of data obtained from comparison of conventional vs. PH microinjected with IKMC ESC clones. GLT (germline transmission) is defined as offspring being paternally derived from the introduced ESC as determined by coat color for conventional host derived animals or by SNP genotyping for PH derived animals. Approximately 50% of germline transmission offspring contained the modified allele (data not shown). With this set of 11 ESC clones germline transmission was obtained with 6/11 using conventional host blastocysts vs 9/11 using PH blastocysts as ESC recipients. If we consider only the first litters then using conventional host, 35% (83/240) of offspring in the first litters were donor ESC germline. In comparison, 100% (137/137) of PH males offspring in the first litters were donor germline.$Fertile refers to the number of PH putative chimeras which proved to be fertile.*Two ESC lines produced PH chimeric males which were subfertile, producing no offspring by natural mating, however they were found to have sperm in the epididymis which were used post cryopreservation for IVF, providing successful germline transmission.#The HEPD0654_5_E11 ESC line microinjected into PH gave germline transmission however, many of the pups, both carrying the modified or wild type allele were born dead or died shortly afterwards. However, survivors carrying the modified allele were obtained after a few litters.