Abrogation of esophageal carcinoma development by miR-31 genetic knockout

Transcriptomics analyses in these Zn-deficient rats revealed the molecular basis of ESCC abrogation by miR-31 knockout: Egln3, a negative regulator of NF-FB, was shown to be a direct miR-31 target; miR-31 inhibition/deletion resulted in suppression of miR-31-associated-EGLN3-NF-KB controlled inflammatory pathways. Genome-wide expression profiling and the analysis of genes with derepressed transcript levels in response to antimiR-31 delivery in vivo. The experimental design consist of rats that received antimirR over the 5 weeks (ZD-antimir31 5weeks), 20 weeks (ZD-antimir31 20 weeks) and untreated rats (ZD:CTRL, ZS: CTRL). Transcriptome profiling was also performed from group of rats with constitutive miR-31 KO (ZD-mir31KO, ZS-mir31KO).