遇见数据集

Data from: A suite of potentially amplifiable microsatellite loci for ten reptiles of conservation concern from Africa and Asia

收藏
DataONE2016-04-06 更新2024-06-26 收录
数据链接:
官方服务:

资源简介:

Here we document the addition of thousands of potentially amplifiable microsatellite loci (PALs) and associated primer sequences for public use. We conducted whole-genome shotgun sequencing to obtain ultra-low coverage, random genomic sampling from ten African and Asian squamate reptile species (representing ten genera). We used unique genomic processing methods and generated PALs for the following species: Acrochordus granulatus, Ahaetulla prasina, Cerberus rhynchops, Gonocephalus kuhlii, Ophiophagus hannah, Python bivittatus, Tribolonotus gracilis, Trimeresurus sabahi (Popeia sabahi), Uromastyx geryi and Varanus exanthematicus. All taxa included, as well as other related taxa not included in our study, are exploited heavily by the international skin and pet trades, yet researchers and conservation agencies currently lack substantial genetic resources for guiding conservation and management. Using stringent filtering methods, we generated between 467 and 8641 PALs for each of the ten species (52,164 PALs total), yielding a rich database of microsatellite loci and amplification primers for these taxa. In addition to the ten species targeted in our study, microsatellite markers provided in this database can likely be applied to a variety of closely related taxa that are also of conservation and commercial interest.

本研究公开了数千条潜在可扩增微卫星位点(Potentially Amplifiable Microsatellite Loci,以下简称PALs)及其配套引物序列,供公众使用。本研究采用全基因组鸟枪测序技术,对10个隶属于10个属的非洲和亚洲鳞龙类爬行动物物种进行了超低覆盖度的随机基因组采样。本研究采用独特的基因组处理方法,为以下物种开发了PALs:Acrochordus granulatus、Ahaetulla prasina、Cerberus rhynchops、Gonocephalus kuhlii、Ophiophagus hannah、Python bivittatus、Tribolonotus gracilis、Trimeresurus sabahi (Popeia sabahi)、Uromastyx geryi及Varanus exanthematicus。所有纳入本研究的类群,以及本研究未涵盖的其他近缘类群,均遭到国际皮肤贸易与宠物贸易的大规模开发,但当前研究人员与保护机构仍缺乏充足的遗传资源以指导其保护与管理工作。本研究通过严格的过滤流程,为10个物种各生成了467至8641条PALs,总计52164条,构建了涵盖这些类群的微卫星位点与扩增引物的丰富数据库。除本研究靶向的10个物种外,本数据库提供的微卫星标记大概率可应用于多种同样兼具保护价值与商业价值的近缘类群。

创建时间:
2016-04-06
二维码
社区交流群
二维码
科研交流群
商业服务