Fast Repetition Rate Fluorometry (FRRF) data collected on the RV L'Astrolabe from 26/02/2005 to 03/03/2005 (Dumont d'Urville - Hobart)

These data were collected on the RV L'Astrolabe (platform code: FHZI) from 26/02/2005 to 03/03/2005 on a trip from Dumont D'Urville to Hobart.

Maximum photochemical efficiency of photosystem II (PSII), also called maximum quantum yield of PSII (Fv/Fm), has become one of the most widely utilized fluorescence parameters in phytoplankton research. It represents the potential photochemical efficiency, which is the probability that the light energy captured by the photosynthetic apparatus is being utilized as photochemistry. Fv/Fm has been shown to have an instant response to variations in physical and chemical properties and is interpreted as a diagnostic of the overall health or competence of phytoplankton. Together with the absorption cross section area of PSII and chlorophyll concentration, it can be used to measure primary production (Cheah et al. 2011, Deep Sea Research).

Seawater from 3 m depth was supplied continuously from the ship’s clean seawater line. FRR fluorescence yields were measured continuously at 1 minute intervals in dark-adapted state (! 15 minutes dark-adaptation) using a flash sequence consisting of a series of 100 subsaturation flashlets (1.1 μs flash duration and 2.8 μs interflash period) and a series of 20 relaxation flashlets (1.1 μs flash duration and 51.6 μs interflash period).

本数据集采集自星盘号（RV L'Astrolabe，平台编码：FHZI），采集时段为2005年2月26日至2005年3月3日，航线为从迪蒙·迪尔维尔（Dumont D'Urville）前往霍巴特（Hobart）。

光合系统II（Photosystem II, PSII）的最大光化学效率，又称PSII最大量子产额（Fv/Fm），是浮游植物研究中应用最广泛的荧光参数之一。该指标表征潜在光化学效率，即光合装置捕获的光能用于光化学反应的概率。研究证实，Fv/Fm可对物理化学性质的变化产生即时响应，可作为浮游植物整体健康状态或生存能力的诊断依据。结合PSII吸收截面积与叶绿素浓度，该指标可用于初级生产力测定（Cheah等，2011，《深海研究》（Deep Sea Research））。

研究采用船舶清洁海水管路连续抽取3米水深的海水。在暗适应（至少15分钟暗适应）状态下，以1分钟间隔连续测定快速重复荧光（Fast Repetition Rate, FRR）产率，所用闪光序列包含两组：100次亚饱和微闪（单次闪光时长1.1μs，闪间间隔2.8μs），以及20次弛豫微闪（单次闪光时长1.1μs，闪间间隔51.6μs）。