Intestinal macrophages modulate synucleinopathy along the gut-brain axis

We generated a digital spatial profiling (DSP) dataset from duodenal myenteric plexus of 3-month-old 3KL and WT mice, analyzing 2–3 ROIs per slide across 2 animals per genotype. The dataset comprises expression values for 25 proteins, including PD-associated targets, lysosomal and autophagy markers, internal housekeeping and background controls. Normalized digital counts were obtained per ROI, enabling comparative analysis of protein signatures between genotypes at an early stage of pathology , , # Intestinal macrophages modulate synucleinopathy along the gut-brain axis Dataset DOI: [10.5061/dryad.12jm63zb6](10.5061/dryad.12jm63zb6) ## Description of the data and file structure The dataset was generated using digital spatial profiling of duodenal myenteric plexus from 3-month-old 3KL and WT mice to capture early molecular changes in enteric synucleinopathy. These data were collected to assess how early α-synuclein pathology correlates with lysosomal stress in the ENS. 5 μm thick FFPE sections of the myenteric plexus were deparaffinized and incubated with antibodies against bIII-tubulin to select myenteric ganglia as segmented ROI, according to the manufacturer’s instructions. Cell nuclei were stained with DAPI. Each ROI was manually drawn around myenteric ganglia in the myenteric plexus (geometric segments), encompassing both neurons and glial cells, resulting in a total of 2-3 analysed ROIs per slide. Each slide was incubated with an antibody panel containing antibodies con...,我们从3月龄3KL与野生型（Wild Type, WT）小鼠的十二指肠肌间神经丛中构建了数字空间图谱分析（digital spatial profiling, DSP）数据集，针对每一种基因型的2只动物，每张切片分析2~3个感兴趣区域（Region of Interest, ROI）。该数据集包含25种蛋白质的表达值，涵盖帕金森病（Parkinson's Disease, PD）相关靶点、溶酶体与自噬标志物、内参管家基因对照及背景对照。每个感兴趣区域均可获得标准化数字计数，可用于比较不同基因型在病理早期的蛋白质特征。 # 肠道巨噬细胞沿肠-脑轴调控突触核蛋白病 数据集DOI：10.5061/dryad.12jm63zb6 ## 数据与文件结构说明 本数据集通过对3月龄3KL与WT小鼠的十二指肠肌间神经丛开展数字空间图谱分析构建，旨在捕捉肠内突触核蛋白病的早期分子变化。本数据用于评估肠神经系统（Enteric Nervous System, ENS）中早期α-突触核蛋白（α-synuclein）病理与溶酶体应激的相关性。 将肌间神经丛的5 μm厚福尔马林固定石蜡包埋（Formalin-Fixed Paraffin-Embedded, FFPE）切片脱蜡后，按照制造商说明书，使用抗βIII-微管蛋白（βIII-tubulin）的抗体孵育，以选取肌间神经节作为分割后的感兴趣区域。细胞核用4',6-二脒基-2-苯基吲哚（4',6-Diamidino-2-phenylindole, DAPI）染色。每张切片的感兴趣区域均手动圈绘于肌间神经丛的肌间神经节周围（几何分割区域），涵盖神经元与神经胶质细胞，最终每张切片共分析2~3个感兴趣区域。每张切片均使用包含抗体的抗体组合进行孵育……