In vitro percutaneous absorption data for model chemicals in Rhinella marina: chemicals formulated in penetration enhancers ethanol or propylene glycol

Previous studies by the authors measured in vitro absorption kinetics of model chemicals through Rh. marina skin when the chemicals were formulated as saturated solutions in Amphibian Ringers Solution. However, many chemicals are formulated in solutions containing penetration enhancers. No data exists to quantify the effect of penetration enhancers on absorption kinetics through frog skin, nor is there information of how these enhancers effect frog skin structure. This dataset provides in vitro absorption kinetics for three model chemicals - benzoic acid, caffeine, and ibuprofen – formulated in either 1/10/30% v/v ethanol or 20% PG solution. Franz-type diffusion cells were used to measure absorption, and chemical content was quantified using validated HPLC methods. Additionally, the impact on frog skin due to exposure to these penetration enhancers was characterised using differential scanning calorimetry (DSC) and histology. Each chemical was formulated as a saturated solution (infinite dose) in one of the enhancers and applied to the external skin surface. Diffusion through the skin was measured over a period of four to six hours. Chemical flux was determined from the steady-state slope of the cumulative absorption vs time plot. Skin samples for DSC experiments and histology were either freshly excised, or exposed to one of the following for a period of six hours: ARS, 1/10/30% v/v ethanol, 20% v/v PG. Data includes a spreadsheet (comma-separated values / .csv file) providing individual animal information (including animal ID, sex, weight, and from which body regions each skin sample was obtained), penetration enhancer identification, chemical information (including name and concentration) and absorption data including chemical content at each sample time and calculated flux and Kp values. DSC and histology are available by contacting the author. Column headings for the spreadsheet are explained below: AnimalID: individual animal identifier Sex: animal sex Weight: frog weight in grams Region: primary skin region (dorsal=D/ ventral=V) of skin sample SubRegion: Identifies if ventral samples are thoracic (T) or pelvic (P) in origin. Dorsal samples remain coded as “D” LeftRightMid: identifies if samples taken from left (L), right (R) or on the midline (M) Solvent: enhancer used (1/10/30% v/v ethanol or 20% PG) Chemical: chemical applied to the skin (caffeine/benzoic acid/ibuprofen) Conc: concentration of chemical applied (microgram/ml) Flux: steady-state flux of the chemical Kp: permeability coefficient of the chemical (Kp=Conc/Flux) t=0 through t=6: concentration (microgram/ml) of chemical in receptor fluid at each sampling time (hr)

本研究团队既往的研究中，测试了模式化学物以两栖林格液（Amphibian Ringers Solution）配制的饱和溶液形式，通过Rh. marina皮肤的体外吸收动力学。然而，多数化学物的制剂会添加促渗剂，目前尚无研究量化促渗剂对蛙皮吸收动力学的影响，也未明确此类促渗剂对蛙皮结构的影响。
本数据集涵盖3种模式化学物——苯甲酸、咖啡因与布洛芬——分别以1/10/30%体积分数乙醇或20%丙二醇（PG）溶液配制时的体外吸收动力学数据。实验采用Franz型扩散池进行吸收量测定，化学物含量通过经过验证的高效液相色谱（HPLC）方法定量。
此外，本数据集还通过差示扫描量热法（DSC）与组织学方法，表征了此类促渗剂暴露对蛙皮的影响。每种化学物均以对应促渗剂中的饱和溶液（无限剂量）形式配制，并施用于皮肤外表面。皮肤渗透过程的采样时长为4至6小时。化学物的通量通过累积吸收量-时间曲线的稳态斜率计算得到。
用于DSC实验与组织学分析的皮肤样本，要么为新鲜离体样本，要么经以下试剂暴露6小时：两栖林格液（ARS）、1/10/30%体积分数乙醇、20%体积分数丙二醇。
数据集包含一份逗号分隔值（CSV）格式的电子表格，其中涵盖个体动物信息（包括动物编号、性别、体重，以及皮肤样本的取材部位）、促渗剂信息、化学物信息（包括名称与浓度），以及吸收相关数据——包括各采样时间点的化学物含量、计算得到的通量与渗透系数（Kp）数值。差示扫描量热法与组织学相关数据可通过联系作者获取。
该电子表格的列标题说明如下：
AnimalID：个体动物唯一标识符
Sex：动物性别
Weight：蛙体重（单位：克）
Region：皮肤样本的主要取材部位（背部=D/腹部=V）
SubRegion：标注腹部样本的取材亚区（胸部=T/骨盆=P），背部样本统一编码为"D"
LeftRightMid：标注样本取材于左侧（L）、右侧（R）还是中线（M）
Solvent：所用促渗剂（1/10/30%体积分数乙醇或20%丙二醇）
Chemical：施用于皮肤的化学物（咖啡因/苯甲酸/布洛芬）
Conc：施用化学物的浓度（单位：微克/毫升）
Flux：化学物的稳态通量
Kp：化学物的渗透系数（Kp=浓度/通量）
t=0至t=6：各采样时间点（单位：小时）受体液中的化学物浓度（单位：微克/毫升）