The EBV microRNA targetome

In EBV-associated tumors, such as gastric cancer (GC) and Burkitt's lymphoma (BL), a high proportion of microRNAs are virally encoded. To explore the targets of both viral and host microRNAs, we performed Crosslinking, Ligation, and Sequencing of Hybrids (CLASH). With this approach, we were able to quantify each Argonaute-bound microRNA-mRNA interaction in a GC (SNU719), and BL (Akata) cell line. Overall design: CLASH analysis of Akata and SNU719 cells, 3 biological replicates of each. miRNA-seq of Akata and SNU719 cells, 3 biological replicates of each.