Substrates are shown aligned as annealed. A boldface and underlined nucleotide indicates a 3’-fluorescent label; 3′C is labeled with FAM, 3′A with ROX. SBO substrates combine both the FAM and ROX frag
aM designates 5-methylcytosine (5mC), H designates 5-hydroxymethylcytosine (5hmC), P designates pyrrolocytosine. Modified bases and mismatched base pairs are shown in boldface, recognition sequences a
aM designates 5-methylcytosine (5mC), H designates 5-hydroxymethylcytosine (5hmC), P designates pyrrolocytosine. Modified bases and mismatched base pairs are shown in boldface, recognition sequences a
Substrates are shown aligned as annealed. A boldface and underlined nucleotide indicates a 3’-fluorescent label; 3′C is labeled with FAM, 3′A with ROX. SBO substrates combine both the FAM and ROX frag