Genome-wide transcripts in reprogramming cells expressing Osr2

Analysis of reprogrammed MEFs expressing Osr2. Results provide insight into an importance of Osr2 downregulation during the reprogramming. Overall design: Total RNA were isolated from reprogrammed MEFs expressing Osr2 or kusabira orange treated with or without 10 µM SB431542.Libraries were sequenced on an Illumina NovaSeq 6000. Sequence reads were mapped to the mouse genome (GRCm38.p6) using STAR aligner (v2.7.3a) with default parameters. The read counts were calculated by featureCounts (v2.0.0) with the gene model from Ensemble (Mus_musculus GRCm38.75.gtf) at gene level to obtain TPM (Transcripts Per Million).