RNAi components regulate the micoparasitism in Trichoderma atroviride

Trichoderma atroviride is a fungus capable of establishing symbiotic relationships with plants, however, its main lifestyle is a saprophyte. Due to these characteristics, it must face a great quantity of microorganisms, and be able to compete for nutrients. T. atroviride is considered a necrotrophic mycoparasite and has developed the ability to kill other organisms and obtain nutrients from them. The object of this work is to explore the role of small RNAs in mycoparasitism. To this end, we obtained RNA-Seq libraries from the interactions of T. atroviride against Alternaria alternata and Rhizoctonia solani. The transcriptomes were obtained from three stages during mycoparasitsm: before contact (BC), contact (C), and after contact (AC). T. atroviride WT vs Rhizoctonia solani AG2; T. atroviride WT vs R. solani AG5; T. atroviride WT vs Alternaria alternata; T. atroviride control T. atroviride dcr2 mutant vs Rhizoctonia solani AG2; T. atroviride dcr2 mutant vs Rhizoctonia solani AG5; T. atroviride dcr2 mutant vs Alternaria alternata; T. atroviride dcr2 mutant control T. atroviride ago3 mutant vs Rhizoctonia solani AG2; T. atroviride ago3 mutant vs Rhizoctonia solani AG5; T. atroviride ago3 mutant vs Alternaria alternata; T. atroviride ago3 control 3 replicates per condition, The controls are T. atroviride strains growing alone without any fungal prey