Transcriptional analysis of DENV-elicited inflammation following experimental human DENV-3 challenge

The objective of this analysis was to determine the transcriptional signature associated with experimental primary DENV-3 infection in human volunteers. Nine flavivirus naive volunteers were challenged with an attenuated DENV-3 strain - CH53489 - and blood collected for RNA extraction and transcriptional analysis on or around study days 0, 6, 8, 10, 14, and 28 post challenge using PAXgene collection tubes. Total RNA was isolated from the collection tubes and subjected to RNAseq analysis to identify genes and gene sets that were differentially expressed across the infection time course. Whole blood was collected on or around days 0, 6, 8, 10, 14, and 28 post DENV-3 (strain CH53489) infection 9 subjects using Paxgene tubes.