ChIP-seq analysis of Rad21 binding during genome activation. Danio rerio

The formation of a transcriptional competent zygote is a not fully understood process potentially involving changes in chromatin structure. Cohesin and CTCF are important nuclear architectural proteins that contribute to chromatin structure and gene regulation. Here we analyze the genome-wide location of Rad21 binding during zebrafish embryogenesis and show increased Rad21 binding over developmental time (2.5 hpf, 4.5 hpf , 10 hpf). Overall design: ChIP-seq to generate genome wide binding data for Rad21 in three stages of zebrafish development, two biological replicates for each stage with input controls.