Oenothera Section Calylophus population genetic study

Premise: Animal pollinators play an important role in pollen dispersal. Differences in foraging patterns, flight distances and grooming behaviors are assumed to have consequences for genetic diversity of plants but are rarely tested explicitly. Here, we assess the role of pollinator functional groups with different foraging behaviors (hawkmoth and bee) in generating patterns of genetic diversity over similar geographic ranges for two closely related taxa. Methods: This study focuses on two members of Oenothera section Calylophus that co-occur on gypsum outcrops throughout the Chihuahua Desert but differ in floral phenotype and primary pollinator: Oenothera gayleana (bee) and O. hartwegii subsp. filifolia (hawkmoth). We measured breeding system and floral traits in the greenhouse and conducted a population genetic study at the local (&lt;13km; four populations) and landscape (60–440km; five populations) scales using 10–11 nuclear (pollen dispersal) and three plastid (seed dispersal) micr..., Floral morphology and leaf tissue for genetic samples were collected in wild populations in New Mexico and Texas, USA. Floral morphology data and the breeding system assessment were conducted on plants grown in a growth chamber at the Chicago Botanic Garden, Illinois, USA. Additional details of methods used can be found in the published manuscript (to be updated upon publication).,研究背景： 传粉动物在花粉扩散过程中发挥关键作用。传粉者的觅食模式、飞行距离及梳理行为的差异被认为会对植物的遗传多样性产生影响，但此类关联鲜有得到明确验证。本研究针对两个近缘类群，在相似地理范围内，探究具有不同觅食行为的传粉功能群（pollinator functional groups）——天蛾（hawkmoth）与蜂类（bee）——在塑造植物遗传多样性格局中的作用。 研究方法： 本研究聚焦于奇瓦瓦沙漠（Chihuahua Desert）石膏露头生境中共存的两个月见草属（Oenothera）卡柳叶组（Calylophus）类群，二者花部表型与主要传粉者存在差异：Oenothera gayleana（主要传粉者为蜂类）与O. hartwegii subsp. filifolia（主要传粉者为天蛾）。本研究于温室中测定了繁育系统（breeding system）与花部性状（floral traits），并基于10至11个核基因位点（用于示踪花粉扩散）与3个质体基因位点（用于示踪种子扩散）的分子标记，分别在局域尺度（local scale，<13km，包含4个种群）与景观尺度（landscape scale，60–440km，包含5个种群）开展了种群遗传学研究。 遗传样本的花部形态数据与叶片组织采自美国新墨西哥州与得克萨斯州的野生种群。花部形态数据采集与繁育系统评估则针对在美国伊利诺伊州芝加哥植物园（Chicago Botanic Garden）生长箱中培育的植株开展。 本研究使用的实验方法的更多细节可参见已发表手稿（将在正式出版后更新）。