Analysis of inter-chromosomal rDNA-associated contact changes in K562 erythroleukemia cells after erythroid differentiation caused by incubation in the presence of hemin [4C]

Expression of the clusters of rDNA genes influences pluripotency, but the underlying mechanisms are not known yet. The clusters shape inter-chromosomal contacts with numerous genes controlling differentiation in human cells. The fact suggests the possible role of these contacts in formation of 3D chromosomal structures and regulation of gene expression in development. We used human leukemia K562 cells induction to erythroid differentiation in order to study the genome-wide changes in inter-chromosomal rDNA-associated contacts. Comparative analysis of genome-wide inter-chromosomal rDNA-associated contact changes by 4C experiments for K562 cells untreated and incubated for 108h with 50 μM concentration of hemin.