Identification of miR-100 and miR-125b targets by AGO2 RIP-seq and RNA-seq after ectopic expression of miR-100 or miR-125b and evaluation of the TGFb expression signature in PANC-1 cells by RNA-seq

To identify targets post-transcriptionally regulated by miR-100 and miR-125b, we have performed AGO2 RIP-seq and RNA-seq following overexpression of the two miRNAs in PANC-1 cells. In addition, we have carried out RNA-seq following TGFb stimulus to evaluate changes in gene expression driven by TGFb. For AGO2 RIP-seq and RNA-seq PANC-1 cells were transfected with precursor miRNA mimics for miR-100, miR-125b and negative control (0.5nM) for 24h. The experiment was performedin two biological replicates. For evaluation of changes in gene expression driven by TGFb PANC-1 cells were treated with TGFb (5nM) or vehicle control for 72h. Three biological replicates for each condition were used for expression analysis.