Thirty two of the deletions identified by the screen.

4773 isogenic MATα deletion strains, each deleted in a single non-essential gene [62], were applied robotically to semi-synthetic medium containing either 0.1% C16:1, 0.1% C18:1, or neither, and grown 2 weeks at 25° [9]. Of the total of 212 strains identified as inhibited (↓) or stimulated (↑) in growth to varying degrees (the greater the effect, the more arrows) by C16:1 or C18:1, growth of 130 showed substantial differential effects of C16:1 and C18:1 on growth (47 were C16:1-inhibited and unaffected by C18:1, 7 were C16:1-inhibited and C18:1-stimulated, 52 were C18:1-inhibited and unaffected by C16:1, 3 were C18:1-inhibited and C16:1-stimulated, 19 were stimulated in growth by C16:1 yet unaffected by C18:1, and 2 were stimulated in growth by C18:1 yet unaffected by C16:1). The growth of 71 of the remaining 82 was inhibited by both C16:1 and C18:1 (37 of these were equally sensitive to both) and the final 11 were stimulated in growth by both fatty acids. See Table S1 for the complete data set.