Smad4 restricts injury-provoked biliary proliferation and carcinogenesis (Smad4_floxed_vs_WT_RNAseq)

To understand the processes regulated by Smad4 in reactive cholangiocytes, Ctl and Smad4del mice were provided DDC diet for 2 weeks after which reactive cholangiocytes were isolated from digested injured mouse liver via FACS followed by RNA amplification and sequencing analysis. Gene set enrichment analysis of Smad4-perturbed reactive cholangiocytes implicates Smad4 in proliferative, metabolic, and inflammatory pathways. Overall design: Comparative gene expression profiling analysis of RNA-seq data for reactive cholangiocytes with and without Smad4