Gene expression data from RT112 following FGFR3 knock-down using FGFR3 siRNAs. Homo sapiens

To better understand the molecular mechanisms underlying altered-FGFR3 oncogenic activity in bladder carcinomas, we made use of RT112 cell lines, which were derived from a human bladder tumor and endogenously expressed the FGFR3-TACC3 fusion protein, the growth and transformation of these cell lines being dependent on activated-FGFR3 activity. We conducted a gene expression analysis using Affymetrix DNA arrays in this cell line treated or not with FGFR3 siRNAs. Overall design: RT112 cells were transfected or not with siRNA FGFR3. mRNA was extracted after 40h and gene expression analysis was assessed using the HG-U133 Plus 2.0 array (Affymetrix) array