Nervous necrosis virus (NNV) detection results from infected grouper fish from 3 different fish farms.

aLocations of the grouper fish farms are shown in Figure S2 and the fish samples were collected in 2008 and 2009. b+, NNV-positive samples by microfluidic chip analysis; −, NNV-negative samples by microfluidic chip analysis. cThe first observation of viral nervous necrosis (VNN) clinical signs following sampling; +, groupers displayed VNN clinical signs; −, groupers did not display any clinical signs of disease; Clinical signs of VNN in larval-stage groupers were abnormal schooling and swimming behavior (whirling, spiraling) and loss of appetite. dThe tracking observation (disease outbreak) 2 weeks after the first observation from the same grouper fish farms; +, groupers displayed VNN clinical signs; −, groupers did not display any clinical signs of disease.