Experimental data for Pseudomonas aeruginosa from experimental evolution under different bottleneck sizes and antibiotic selection pressures

We here combined evolution experiments with genomic and genetic analyses to assess whether bottleneck size and antibiotic-induced selection influences the evolutionary path to resistance in pathogenic Pseudomonas aeruginosa, one of the most problematic opportunistic human pathogens. Two sets of evolution experiments were performed across 16 transfers, in which either the aminoglycoside gentamicin or the fluoroquinolone ciprofloxacin were used as antibiotic. The evolutionary response was studied using counts of bacterial cells at the end of each transfer period (i.e., yield) or by calculating the growth rate from regular optical density measurement during the respective transfer periods. For the populations at the end of the evolution experiments, we also determined their antibiotic resistance with the help of standardized dose response curves. Moreover, we performed whole genome sequencing to assess the frequency of variants, which emerged and spread during the evolution experiments. We...本研究结合进化实验与基因组、遗传分析，旨在评估瓶颈大小与抗生素诱导选择是否会影响条件致病性人类病原体——铜绿假单胞菌（Pseudomonas aeruginosa）的耐药性进化路径，该菌是临床最棘手的机会致病菌之一。本研究开展了两组共16轮传代的进化实验，分别以氨基糖苷类抗生素庆大霉素（gentamicin）与氟喹诺酮类抗生素环丙沙星（ciprofloxacin）作为筛选药物。通过每轮传代末期的细菌细胞计数（即菌量产量），或是通过对应传代期间的常规光密度检测计算生长速率，以分析菌群的进化应答情况。针对进化实验结束后的菌群种群，本研究还通过标准化剂量反应曲线测定了其抗生素耐药性水平。此外，本研究开展了全基因组测序，以评估进化实验过程中出现并扩散的变异位点的频率。本研究……