GUIDE-seq

NIAID Data Ecosystem2026-05-01 收录

基因组编辑脱靶检测

DSB定位

数据链接：

https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1043859 数据链接链接失效反馈

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资源简介：

genome-wide, unbiased identification of DSBs enabled by sequencing, provides an unbiased, genome-wide and sensitive method for detecting RGN-induced DSBs

创建时间：

2023-11-22

Detecting off-target mutations in genome-editing by nuclear transfer and sequencing

基因组编辑脱靶检测

细胞突变分析

detecting the off-target mutations in genome-editing in any clonal cells

DataCite Commons2021-05-08 更新80

Additional file 3: of NmeCas9 is an intrinsically high-fidelity genome-editing platform

基因组编辑脱靶检测

Cas9蛋白

Table S2. Summary of potential off-target sites detected by GUIDE-seq analysis using the Bioconductor package GUIDE-seq. The table includes sites for both NmeCas9 (with N4GN3 PAM sequences) and SpyCas

Figshare2024-02-08 更新70

Additional file 10: of NmeCas9 is an intrinsically high-fidelity genome-editing platform

基因组编辑脱靶检测

Cas9核酸酶特异性评估

Table S3. Targeted deep sequencing read count data for all sgRNAs used and for all off-targets detected by GUIDE-seq. (XLSX 44 kb)

Figshare2024-02-08 更新60

Cytosine base editors induce prevalent unwanted out-of-protospacer editing and target-strand editing [Targeted amplicon sequencing]

基因组编辑脱靶检测

碱基编辑器安全性评估

The Detect-seq provides an unbiased method for genome-wide identification of CBE induced off-targets inside of cells. Purpose: Cytosine base editors (CBEs) have the potential to correct human path

NIAID Data Ecosystem70

Detect-seq on transfected HEK293T cells [Detect-seq data]. Detect-seq on transfected HEK293T cells [Detect-seq data]

基因组编辑脱靶检测

线粒体DNA编辑评估

We used a new technology, named Detect-seq, to perform genome sequencing on transfected HEK293T cells to see DdCBEs' off-target mutations. Besides, targeted-amplicon sequencing and ATAC-seq data were

NIAID Data Ecosystem70

GUIDE-seq

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