rRNA gene sequencing of the gastric microbiota of mice

The goal of the rRNA gene sequencing was two-fold: 1) to assess potential effects of the gastrin transgene on the gastric microbiota in INS-GAS mice and 2) to address the potential effects of iron deficiency on the gastric microbiota in INS-GAS mice. Collectively, the rationale of this work was to assess the contribution of the gastric microbiota in the development of precancerous lesions that occur in INS-GAS mice due to the presence of the gastrin transgene as well as iron deficiency.