Live Imaging of SARS-CoV-2 Infection in Mice Reveals that Neutralizing Antibodies Require Fc Function for Optimal Efficacy by Ullah et al_IMMUNITY-D-21-00378

Supplementary videos and raw data set for all the graphs shown in figures the manuscript titled "Live Imaging of SARS-CoV-2 Infection in Mice Reveals that Neutralizing Antibodies Require Fc Function for Optimal Efficacy" by Ullah et al_IMMUNITY-D-21-00378

Supplementary Video Legends:
Video S1. Longitudinal Non-invasive Bioluminescence Imaging to Visualize SARS-CoV-2-nLuc Infection and Dissemination in K18-hACE2 Mice, Related to Figure 1.
SARS-CoV-2-nLuc challenged mice were imaged daily in dorsal (d) and ventral (v) positions for 6 days using IVIS Spectrum to monitor virus spread in the whole body as well as neuroinvasion.

Video S2. Tomographic Reconstruction of Lung Tissue Depicting SARS-CoV-2 Virions in Infected Cells, Related to Figure S2, panels B-D.
Virus particles are found within membrane-enclosed exit compartments of two adjacent pulmonary capillary endothelial cells. The movie traverses the reconstructed volume to illustrate the compartments (red arrowheads) then increases in magnification to detail the virions within the compartments.

Video S3. Cellular Overview and Tomogram of SARS-CoV-2 Infected Region in Lung Tissue with Alveolar Macrophages, Related to Figure S2, panels B-D.
SARS-CoV-2 virions are found in regions containing identifiable immune cells. Movie begins with a large-field montaged overview, highlighting alveolar macrophages (blue), AT2 cells (green), AT1 cells (yellow) and pulmonary blood veins (red). The upper of two blood veins is detailed at higher magnification, showing 3 red blood cells (rbc) and the surrounding capillary endothelium. A region containing portions of two endothelial cells is selected for tomographic reconstruction, showing caveolae at the cell surfaces and localizing SARS-CoV-2 virions within cytoplasmic exit compartments.

Video S4. Tomographic Reconstruction of SARS-CoV-2 Infected Brain Tissue Depicting Infected Neurons, Related to Figure S2, panel F.
Virus particles are found within neurons, often appearing in linear groups within compartments bordering the edges of neuronal projections. The movie details the distinction between presumptive SARS-CoV-2 virions and typical synaptic neurotransmitter vesicles found in an adjacent synaptic terminal.

Video S5. Tomographic Reconstruction of Mouse Testis Depicting SARS-CoV-2 Virions Within Sertoli Cells, Related to Figure S2, panel M.
Virus particles are found within membrane-enclosed compartments of Sertoli cells. Additional material and structures coexist with the virions in these compartments, suggesting they may be defined as lysosomes. Presumptive SARS-CoV-2 virions can be discerned from the other structures.

本数据集对应Ullah等人发表于期刊IMMUNITY-D-21-00378、题为《小鼠体内SARS-CoV-2感染实时成像显示中和抗体需依托Fc功能实现最佳疗效》的手稿中所有配图图表的补充视频与原始数据集。

补充视频说明：
视频S1：纵向无创生物发光成像可视化K18-hACE2小鼠体内SARS-CoV-2-nLuc感染与播散（对应图1）。将经SARS-CoV-2-nLuc攻毒的小鼠连续6天每日于背侧（d）与腹侧（v）体位使用IVIS Spectrum活体成像系统进行成像，以监测病毒在全身的扩散以及神经侵袭情况。

视频S2：肺组织断层重建成像，展示感染细胞内的SARS-CoV-2病毒粒子（对应补充图S2的B-D面板）。病毒颗粒存在于两个相邻肺毛细血管内皮细胞的膜包被排出隔间内。本视频遍历重建体积以展示该隔间（红色箭头标示），随后放大倍率以细化展示隔间内的病毒粒子。

视频S3：肺组织中SARS-CoV-2感染区域的细胞概览与断层成像（对应补充图S2的B-D面板）。SARS-CoV-2病毒粒子存在于可识别免疫细胞的区域内。视频以大视野拼接概览开场，高亮标注肺泡巨噬细胞（蓝色）、肺泡Ⅱ型上皮细胞（AT2细胞，绿色）、肺泡Ⅰ型上皮细胞（AT1细胞，黄色）以及肺静脉（红色）。随后对两条静脉中的上方静脉进行高倍率细节展示，可见3个红细胞（rbc）及其周围的毛细血管内皮。选取包含两个内皮细胞部分区域的位点进行断层重建，展示细胞表面的胞膜窖，并定位SARS-CoV-2病毒粒子于细胞质排出隔间内。

视频S4：感染SARS-CoV-2的脑组织断层重建成像，展示受感染神经元（对应补充图S2的F面板）。病毒颗粒存在于神经元内，常以线性簇状分布于神经元突起边缘的隔间中。本视频详细区分了疑似SARS-CoV-2病毒粒子与相邻突触末端内的典型突触神经递质囊泡。

视频S5：小鼠睾丸断层重建成像，展示支持细胞（Sertoli细胞）内的SARS-CoV-2病毒粒子（对应补充图S2的M面板）。病毒颗粒存在于支持细胞的膜包被隔间内。这些隔间内与病毒粒子共存的额外物质与结构提示其可能为溶酶体，可将疑似SARS-CoV-2病毒粒子与其他结构区分开来。