Data S1 - Unexplored Character Diversity in Onychophora (Velvet Worms): A Comparative Study of Three Peripatid Species

Supplementary file containing the following figures and tables.: Figure S1. Eversible coxal vesicles (arrowheads). (A) Principapillatus hitoyensis gen. et. sp. nov. Light micrograph of ventral leg surface. (B) Eoperipatus sp.. Scanning electron micrograph of ventral leg surface. Anterior is right in both images. Figure S2. Additional features of Principapillatus hitoyensis gen. et sp. nov. (A) Characteristics of the inner and outer jaw blades. (B) Arrangement of transverse rings on legs. Anterior is left. Note the presence of thin semi-rings (arrowheads) between the complete rings (white dots). Circular inset shows an enlarged primary papilla. Abbreviations: at, accessory tooth; be, bean-shaped papillae; dt, denticles; ib, inner jaw blade; ob, outer jaw blade; pt, principal tooth. Figure S3. Number of births during the lifespan in four females of Principapillatus hitoyensis gen. et sp. nov. Lifespan is represented by horizontal lines; number of births is illustrated by vertical bars. The left and right filled circles associated with horizontal lines indicate birth and death of each female, respectively. Figure S4. Maximum Likelihood topology illustrating the phylogenetic relationships of several species of Peripatidae. Combined analysis of nucleotide sequences of 12S rRNA and translated aminoacids of COI, with five peripatopsid species as an outgroup. Bootstrap values lower than 50 are not shown. Abbreviations correspond to the accession numbers of the COI sequence in GenBank. Figure S5. Karyotype of Eoperipatus sp. Inset shows a light micrograph of the original preparation of mitotic chromosomes from a testis stained with Giemsa. Note three pairs of large and one pair of small chromosomes. Table S1. Number of specimens analysed using each method. Numbers are provided for specimens of different sexes and ages, including embryos. Table S2. Current inconsistencies with the diagnostic features used for the peripatid genera. Symbols used are as follows: (+) present, (−) absent, (+/−) found only in some species of the genus, (?) data unavailable. Grey shading highlights potentially unique features for each genus (note that most proposed diagnostic features are not unique and have to be revised).
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