Additional file 1 of Microsatellite markers from the 'South American fruit fly' Anastrepha fraterculus: a valuable tool for population genetic analysis and SIT applications

Additional file 1: Basic characteristics and PCR amplification conditions of 50 A. fraterculus microsatellite loci. Detailed legend: N: number of individuals analyzed; Dye: fluorescent label incorporated in forward primers; T: optimized annealing temperature; [MgCl2]: optimized magnesium chloride concentration; Size: size of PCR product predicted by Primer 3 software. A: Number of alleles detected. NA: no PCR amplification observed; ND: number of alleles not determined; MP: multiple peaks or bands observed in agarose gels or capillary electrophoresis analysis; CP: complex peaks: complex pattern of peaks observed in capillary electrophoresis analysis, difficult allele scoring. * Primers designed by GIS Company using Designer PCR software v 1.03 (Research Genetics, Inc.). (XLS 42 KB)

附加文件1：50个南美按实蝇（A. fraterculus）微卫星位点的基本特征与聚合酶链式反应（PCR）扩增条件。详细说明如下：N：分析的个体数；Dye：正向引物上标记的荧光基团；T：优化后的退火温度；[MgCl₂]：优化后的氯化镁浓度；Size：由Primer 3软件预测的PCR产物长度；A：检测到的等位基因数；NA：未观测到PCR扩增；ND：未确定的等位基因数；MP：琼脂糖凝胶或毛细管电泳分析中观测到多峰或多条带；CP：复杂峰：毛细管电泳分析中观测到的复杂峰型，等位基因分型困难；* 引物由GIS公司使用Designer PCR软件v1.03（Research Genetics, Inc.）设计。（XLS格式，42 KB）