Summary of the specificity, binding to alanine E mutants and PRNT50 of 12 mAbs.

*Classes of mAbs include GR (group-reactive), CR (complex-reactive), sCR (subcomplex-reactive) and TS (type-specific). Specificity was determined by Western blot (WB) analysis using lysates derived form DENV1, 2, 3, 4 or JEV-infected C6/36 cells as described in Methods.**Alanine E mutants with consistent reduction in binding by dot blot and WB analyses are shown. ++: severe reduction in binding (reduction in R.I.≥70%);+: moderate reduction in binding (50%≤reduction in R.I.†Different regions (strand, loop, etc.) of domains II and III are defined based on the X-ray structure of DENV E protein [7], [24], [33].‡PRNT50 was presented as the lowest concentration that inhibited ≥50% of plaques [38].