Post-Translational Modification Analysis in TCR Complexes Using Mass Spectrometry

This study investigates the structural and biochemical characteristics of T cell receptors (TCRs) involved in immune recognition. Using mass spectrometry-based proteomics, we analyzed post-translational modifications (PTMs), including glycosylation patterns, of different TCR variants. These modifications provide insight into the molecular regulation of TCR function and their interaction with peptide-MHC complexes. Complementary biophysical analyses, such as mass photometry, were performed to understand the oligomeric states of TCR-pMHC complexes. The findings contribute to a deeper understanding of TCR regulation and immune signaling mechanisms.