RNA-seq profiling of 368T1 murine KP NSCLC cells deleted for AMPK with AMPKa1 add-back subjected to no glucose conditions, Replicate 1

This dataset was generated for the paper, "Genetic analysis reveals AMPK is required to support tumor growth in murine Kras-dependent lung cancer models." Murine Kras mutant, p53 null (KP) 368T1 NSCLC cells were deleted for AMPK using the CRISPR/Cas9 system and subsequently stably infected with control vector ("KO") or AMPKalpha1 cDNA ("A1") add-back. These cells were subjected to no glucose (0mM, "NG") conditions for 12 or 18 hours, and profiled by RNA-sequencing. Replicate 1 High Glucose (HG) and No Glucose (NG) conditions were generated simultaneously and analyzed together. Analysis of a single dataset was performed using Replicate 1 ("R1"), and both replicates ("R1" and "R2") were analyzed together as indicated.

本数据集为论文《遗传分析表明腺苷酸活化蛋白激酶（AMPK）在小鼠KRAS依赖性肺癌模型中支持肿瘤生长所必需》所生成。研究人员通过CRISPR/Cas9基因编辑系统，对小鼠KRAS突变、p53缺失（KP）的368T1非小细胞肺癌（NSCLC）细胞完成AMPK敲除，随后分别稳定感染对照载体（标记为"KO"）或AMPKα1 cDNA（标记为"A1"）以实现基因回补。将上述细胞置于无糖（0mM，标记为"NG"）培养环境中处理12或18小时，随后通过RNA测序（RNA-seq）进行转录组分析。同步构建第1次生物学重复的高糖（HG）与无糖（NG）条件样本，并将两组样本合并分析。部分分析仅使用第1次重复样本（R1）完成，其余分析则按要求将两次重复样本（R1与R2）合并开展。