RNA-seq of zinc-replete wild type and zinc-limited wild type, altRP operon deletion mutant and its complement strain in Mycolicibacterium (Mycobacterium) smegmatis

The study was designed to describe the effects of zinc limitation on gene expression in M. smegmatis by comparing the transcriptomes of zinc-replete and zinc-limited wild type. Additionally, the study informs how loss of the altRP operon impacts the transcriptomic changes during zinc limitation by comparing the transcriptomes of zinc-limited ?altRP mutant to the zinc-replete and zinc-limited wild type, and to confirm which changes are complemented in the complement strain (?altRP/c). Overall design: There are 12 samples in total. For each strain (wild type, ?altRP and ?altRP/c) and condition (ie, zinc-replete and zinc-limited) three biological replicates are analyzed.