SNCA targeted gDNA: PacBio raw data

The landscape of SNCA transcripts across synucleinopathies: New insights from long reads sequencing analysis <strong>gDNA capture using IDT xGen</strong><strong>® Lockdown</strong><strong>® Probes and single-molecule sequencing</strong> 2µg of each gDNA sample was sheared to 6kb using the Covaris g-TUBE and ligated with barcoded adapters. An equimolar pool of 12-plex barcoded gDNA library (2µg total) was input into the probe based capture with a custom designed SNCA gene panel. A SMRTBell library was constructed using 626ng of captured and re-amplified gDNA (https://www.pacb.com/wp-content/uploads/Procedure-Checklist-%E2%80%93-Multiplex-Genomic-DNA-Target-Capture-Using-IDT-xGen-Lockdown-Probes.pdf). A total of 3 SMRT Cells (6 hour movie) were sequenced on the PacBio Sequel platform using 2.0 chemistry. Barcodes used for gDNA demultiplexing bc1001: gcagtcgaacatgtagctgactcaggtcacCACATATCAGAGTGCG<br> bc1002: gcagtcgaacatgtagctgactcaggtcacACACACAGACTGTGAG<br> bc1003: gcagtcgaacatgtagctgactcaggtcacACACATCTCGTGAGAG<br> bc1004: gcagtcgaacatgtagctgactcaggtcacCACGCACACACGCGCG<br> bc1005: gcagtcgaacatgtagctgactcaggtcacCACTCGACTCTCGCGT<br> bc1006: gcagtcgaacatgtagctgactcaggtcacCATATATATCAGCTGT<br> bc1007: gcagtcgaacatgtagctgactcaggtcacTCTGTATCTCTATGTG<br> bc1008: gcagtcgaacatgtagctgactcaggtcacACAGTCGAGCGCTGCG<br> bc1009: gcagtcgaacatgtagctgactcaggtcacACACACGCGAGACAGA<br> bc1010: gcagtcgaacatgtagctgactcaggtcacACGCGCTATCTCAGAG<br> bc1011: gcagtcgaacatgtagctgactcaggtcacCTATACGTATATCTAT<br> bc1012: gcagtcgaacatgtagctgactcaggtcacACACTAGATCGCGTGT