Data underlying the publication: Imaging Flow Cytometry for High-Throughput Phenotyping of Synthetic Cells

Data underlying the publication: 1. Godino, E., Restrepo Sierra, A. M. &amp; Danelon, C. Imaging Flow Cytometry for High-Throughput Phenotyping of Synthetic Cells. ACS Synth. Biol. 12, 2015–2028 (2023).<br>The hereby provided research was designed and performed as part of the synthetic biology and BaSyc (NL) consortium efforts to build a synthetic cell from the bottom up. The provided dataset is fraction (100K) of a bigger data set from gene-expressing synthetic vesicles. The data was recorded with The Cytek® Amnis® ImageStream®X Mk II, and analyzed using the IDEAS image analysis software as indicated in the above mentioned publication. Each of the provided files contains the pipeline used for the analysis of the data and can be used as templates for a new analysis. The names of the files indicate the phenotype that was being analyzed, and the figure in the manuscript to which the data belongs to.

本数据集对应发表论文：1. Godino, E., Restrepo Sierra, A. M. 与 Danelon, C. 用于合成细胞高通量表型分析的成像流式细胞术（Imaging Flow Cytometry）. 《ACS合成生物学》（ACS Synth. Biol.）12卷，2015–2028页（2023年）。
本次提供的研究是合成生物学与荷兰BaSyc联盟（BaSyc (NL)）自下而上构建合成细胞项目的组成部分，由该联盟完成设计与实施。
本次提供的数据集为源自基因表达合成囊泡的大型数据集的子集（10万条数据）。
该数据集通过赛塔®（Cytek®）爱姆尼斯®（Amnis®）ImageStream®X Mk II成像流式细胞仪采集，并按照上述发表文献中的方法，使用IDEAS图像分析软件完成数据分析。
每份提供的文件均包含用于该数据集分析的流程管线，可作为全新数据分析的模板使用。
文件名可反映当前分析的表型，以及该数据对应论文中的插图编号。