Investigation on different gene expression of TT19 suppressors under anthocyanin inducing conditions (AIC) using RNA-seq

24 samples of total RNA of 8 Arabidopsis lines (col-0, tt19, and six tt19 suppressors in triplicate) which were under AIC (shaking in 3% sucrose for four days under 24hrs of light) was extracted. mRNA libraries were prepared by BGI company and sequenced BGISEQ-500 as 100PE. 24 samples of total RNAs from 8 Arabidopsis lines (col-0, tt19, and six tt19 suppressors with triplicate) which were under AIC for four days was extracted. The gene differential expression among col-0, tt19, and tt19 suppressors was examined. 8 total genotypes: Col-0 is used as wild type or control; tt19 is a T-DNA insertion line SALK_105779 (under Col-0 background) that knockout TT19 (At5g17220) function; and the six tt19 mutant suppressors are EMS mutagenized point mutation under tt19 background (which is SALK_105779 in Col-0).