The effect of RsaI on global gene expression in S. aureus

The virulon of Staphyloccocus aureus is controlled by intricate connections between transcriptional and post-transcriptional regulators including proteins and small non coding RNAs (sRNAs). Most of the sRNAs regulate gene expression through base-pairings with mRNAs. Here, we have determined the transcriptome of RsaI sRNA of S. aureus. Global overview of RsaI impact on gene regulation, a comparative transcriptomic analysis was performed on total RNAs extracted from the WT HG001 strain, the isogenic HG001∆rsaI mutant strain, and the same mutant strain complemented with a plasmid expressing RsaI under the control of its own promoter