Identification of genes whose expressions are affected by hedgehog RNAi in spider embryos (CBX143). unidentified

This experiment was aimed to discover candidate genes whose expressions might be affected by parental RNA interference (pRNAi) against an Achaearanea tepidariorum homolog of Drosophila hedgehog, At-hh, in spider embryos. Oligonucleotide probes were designed based on accumulated A. tepidariorum EST sequences and embeded in custom microarrays (12K x2). Total RNA was extracted from late stage 5 embryos derived from a mated female before and after injection of dsRNA targeted for At-hh. Gene expression levels were compared between the untreated (normal) and the At-hh pRNAi-treated samples. Probes for At-hh and a homolog of Drosophila orthodenticle, At-otd, served as positive controls.